The adult mouse retina samples were obtained from pool 2 of C57/B6 mice (n = 350), which were purchased from Charles River Laboratories (Charles River Laboratories, Inc., Willmington, MA). All samples were placed in TRIzol reagent (Invitrogen, Carlsbad, CA) and stored at -80ºC before RNA extraction. Total RNA was extracted with TRIzol reagent according to the manufacturer's instructions. For a quality control measure of the samples, total RNA was ran through a 1% agarose gel and using the 2100 Agilent BioAnalyzer System (Agilent Technologies, Palo Alto, CA) to check for possible contamination and degradation.
Extracted molecule
total RNA
Extraction protocol
Retinas were dissected, collected and stored in Trizol (one pair of retinas per eppendorf tube) at -80°C prior to pooling.
Label
Biotin
Label protocol
CodeLink Expression Assay protocol
Hybridization protocol
CodeLink Expression Assay protocol
Scan protocol
CodeLink Expression Assay protocol
Description
MRP2 technical replicate 5
Data processing
Signal intensities across microarrays were normalized using the quantile normalization (www.bioconductor.org).
