|
| Status |
Public on Dec 30, 2006 |
| Title |
LARK_overexpression_adult_head_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Drosophila adult head with overexpressing LARK, ZT6
|
| Organism |
Drosophila melanogaster |
| Characteristics |
Total RNA isolated from adult head of flies overexpressing LARK in their CNS neurons. Sample collected at ZT6.
|
| Biomaterial provider |
Yanmei Huang
|
| Treatment protocol |
Female elav-gal4 flies were mated to male uas-lark flies. Progenies from this cross were frozen at ZT6. Heads were collected from the frozen flies.
|
| Growth protocol |
Fly cultures were grown in 25oC on standard media made from receipt provided by the Bloomington stock center. The culture were entrained to a 12 hour:12 hour light:dark cycle.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNAs were extracted from the head collections using the TriZol reagent from Invitrogen.
|
| Label |
biotin
|
| Label protocol |
Affymetrix one cycle cDNA synthesis kit and and IVT labeling kit were used to label the RNAs following the standard protocols provided by Affymetrix.
|
| |
|
| Hybridization protocol |
Labeled RNAs were hybridized to the Drosophila genome array following standard protocol provided by Affymetrix. The array was washed and stained on a Affymetrix Fluidics Station 400 using standard protocols.
|
| Scan protocol |
An Agilent GeneArray Scanner was used to scan the array.
Pixel size: 3 um, filter: 570nm, number of scan: 2
|
| Description |
The population contain a mixture of elav-gal4; uas-lark/+ male and elav-gal4/+; uas-lark/+ female.
|
| Data processing |
Data generated by MAS5.0 with all default parameters and scaled to a target intensity of 100
|
| |
|
| Submission date |
Dec 03, 2006 |
| Last update date |
Dec 29, 2006 |
| Contact name |
Yanmei Huang |
| E-mail(s) |
[email protected]
|
| Phone |
617-636-3680
|
| Fax |
617-636-2413
|
| Organization name |
Tufts University School of Medicine
|
| Department |
Neuroscience
|
| Lab |
F. Rob Jackson
|
| Street address |
136 Harrison Ave, A305B
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02111 |
| Country |
USA |
| |
|
| Platform ID |
GPL72 |
| Series (1) |
| GSE6420 |
Effect of LARK overexpression in CNS neurons |
|
