|
| Status |
Public on Dec 15, 2006 |
| Title |
MOLT4_ctrl_C |
| Sample type |
RNA |
| |
|
| Source name |
MOLT4 cell line, untreated
|
| Organism |
Homo sapiens |
| Characteristics |
genotype: mutation in the NOTCH 1 gene (Weng et al., 2004, Science, 306, 269-271)
|
| Biomaterial provider |
originally derived from the ATCC stock (CRL-1582)
|
| Treatment protocol |
untreated control
|
| Growth protocol |
RPMI medium 1640 (Invitrogen) supplemented with 10 % FBS, 10 mM HEPES and penicillin-streptomycin (200 U/ml).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
cells were harvested and lysed using Trizol (Invitrogen), with an additional RNA clean-up step using the RNAeasy purification kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip Expression 3’ Amplification One-Cycle Target Labeling kit from about 5ug of total RNA.
|
| |
|
| Hybridization protocol |
standard Affymetrix EukGE-WS2v4 protocol
|
| Scan protocol |
standard Affymetrix procedures using a GeneChip Scanner 3000 7G with autoloader
|
| Description |
MOLT4 cells (a human T-cell acute lymphoblastic leukemia cell line), with a mutation in the NOTCH 1 gene (Weng et al., 2004, Science, 306, 269-271).
Total RNA was harvested from three independent cultures of the MOLT4 cell line before and 48 hours after addition of the gamma-secretase inhibitor DAPT (5 uM).
|
| Data processing |
all probe sets scaling to target signal 100 (GCOS 1.4)
|
| |
|
| Submission date |
Dec 10, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
David Brodin |
| E-mail(s) |
[email protected]
|
| Phone |
+46058583726
|
| URL |
http://www.bea.ki.se
|
| Organization name |
Karolinska Institute
|
| Department |
Department of biosciences and Nutrition
|
| Lab |
Affymetrix lab
|
| Street address |
Halsovagen 7-9
|
| City |
Stockholm |
| ZIP/Postal code |
S-141 57 Huddinge |
| Country |
Sweden |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE6495 |
NOTCH signaling in T-cell acute lymphoblastic leukemia cell lines |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
