|
| Status |
Public on Dec 13, 2006 |
| Title |
Untreated Control 4weeks AffyGC RMA rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Right brain hemisphere ,C57BL male mouse,Un-treated Control,4 weeks
|
| Organism |
Mus musculus |
| Characteristics |
Tissue: Forebrain (left hemisphere), Strain: C57BL, Gender: Male, Age: 4weeks, Drug treatment: Untreated-Control, Period of treatment: 4 weeks, Method of sacrificing: cervical dislocation.
|
| Treatment protocol |
Mouse was housed individually in a separate cage under a 12-h light–dark cycle and permitted food and water ad libitum. A period of acclimatization for two weeks was allowed prior to any experimental procedure. No treatment was added to the animal’s drinking water .The weight and water consumption was measured weekly. At the end of 4 weeks animal was sacrificed by cervical dislocation and the brain was removed, dissected into left and right hemispheres and cerebellum, flash frozen in liquid nitrogen and stored at -80 C for future use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 8-10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Murine Genome U74 Version 2 Set MG-U74A. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner (Agilent Technologies, Foster City, CA)..
|
| Description |
Gene expression data from the right forebrain of C57BL mouse received no treatment for four weeks.
|
| Data processing |
Normalized expression values were calculated from probe level data (CEL file) using the function of Robust Multi-Array expression measure using sequence information GC-RMA (Wu et al 2004, www.bepress.com/jhubiostat/paper4).Computational steps were implemented in the R-language. GCRMA and it's associated packages were provided by the open source bioconductor project.
|
| |
|
| Submission date |
Dec 12, 2006 |
| Last update date |
Dec 12, 2006 |
| Contact name |
Mie A Rizig |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
+44 (0)2076799436
|
| Fax |
+44 (0)2076799437
|
| Organization name |
University Collage London
|
| Department |
Mental Health Sciences
|
| Lab |
Molecular Psychiatry Lab
|
| Street address |
Windeyer Institute of Medical Sciences,46 Cleveland Street
|
| City |
London |
| ZIP/Postal code |
W1T 4JF |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL81 |
| Series (2) |
| GSE6511 |
Four weeks expression data of the antipsychotics Clozapine and Haloperidol in the mouse brain (Affymetrix, GCRMA). |
| GSE6512 |
Clozapine and Haloperidol effect on the mouse brain |
|
