|
| Status |
Public on Sep 06, 2014 |
| Title |
HepG2, siControl, vehicle, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2_siControl_vehicle
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HepG2
transfected with: AllStars Negative Control siRNA
treated with: vehicle
|
| Treatment protocol |
HepG2 cells were transfected with 96nM siRNA for IDE or AllStars Negative Control siRNA (Qiagen) using Lipofectamine 2000 (Invitrogen). 16 h after transfection, cells were treated with 10 nM insulin (Sigma Aldrich) or vehicle for 24 h in serum starvation condition.
|
| Growth protocol |
HepG2 cells were grown in DMEM supplemented with 10% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1 microgram total RNA.
|
| |
|
| Hybridization protocol |
Fragmented cRNA was hybridized to GeneChip Human Gene 1.0 ST Array for 16h at 45° C according to the manufacturer’s instructions. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
Microarrays were scanned with an Affymetrix scanner controlled by Affymetrix Microarray Suite software.
|
| Description |
siCont_k2
|
| Data processing |
The raw fluorescence intensity values were normalized applying quantile normalization.
|
| |
|
| Submission date |
Sep 05, 2014 |
| Last update date |
Sep 06, 2014 |
| Contact name |
Olga Pivovarova |
| E-mail(s) |
[email protected]
|
| Organization name |
German Institute of Human Nutrition
|
| Department |
Clinical Nutrition
|
| Street address |
Arthur-Scheunert-Allee 114-116
|
| City |
Nuthetal |
| ZIP/Postal code |
14558 |
| Country |
Germany |
| |
|
| Platform ID |
GPL15034 |
| Series (1) |
| GSE61142 |
Effects of the insulin degrading enzyme silencing on the transcriptome of HepG2 cells |
|
