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Sample GSM150321

Query DataSets for GSM150321

Status

Public on Dec 15, 2006

Title

earlybird wildtype hippocampus hew2 B

Sample type

RNA

Source name

hippocampus

Organism

Mus musculus

Characteristics

earlybird wildtype hippocampus

Extracted molecule

total RNA

Extraction protocol

Approximately 50 mg of tissue was homogenized in 1 ml TriZol reagent and total RNA was extracted according to manufacture's protocol. Total RNA was cleaned up using an RNeasy kit (Qiagen).

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

hippocampus from wild type littermate of earlybird mutant mouse

Data processing

Affymetrix Microarray Suite version 5.0

Submission date

Dec 14, 2006

Last update date

Dec 14, 2006

Contact name

Maja Bucan

E-mail(s)

[email protected]

Organization name

UPENN

Street address

415 Curie Blvd

City

Philadelphia

State/province

ZIP/Postal code

19104

Country

USA

Platform ID

GPL340

Series (1)

GSE6527

Earlybird mutation and knockout of Rab3a on gene expression in the cortex and hippocampus

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	292.7	P	0.013811
AFFX-BioB-M_at	346.3	P	0.000225
AFFX-BioB-3_at	186.6	P	0.005565
AFFX-BioC-5_at	601.9	P	0.001248
AFFX-BioC-3_at	355.5	P	0.00034
AFFX-BioDn-5_at	481.2	P	0.00011
AFFX-BioDn-3_at	3299.8	P	0.000258
AFFX-CreX-5_at	6555.2	P	0.000044
AFFX-CreX-3_at	8759.3	P	0.000044
AFFX-DapX-5_at	14.3	A	0.440646
AFFX-DapX-M_at	49.6	A	0.262827
AFFX-DapX-3_at	8.3	A	0.973889
AFFX-LysX-5_at	1.5	A	0.981719
AFFX-LysX-M_at	12.4	A	0.860518
AFFX-LysX-3_at	32.3	A	0.216524
AFFX-PheX-5_at	7.4	A	0.910522
AFFX-PheX-M_at	6.3	A	0.868639
AFFX-PheX-3_at	6.8	A	0.953518
AFFX-ThrX-5_at	14.6	A	0.672921
AFFX-ThrX-M_at	14.3	A	0.760937

Total number of rows: 22575

Table truncated, full table size 599 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM150321.CEL.gz	3.3 Mb	(ftp)(http)	CEL