|
| Status |
Public on Sep 30, 2014 |
| Title |
STING tumor DNA |
| Sample type |
RNA |
| |
|
| Source name |
STING knock out BMDCs tumor-derived DNA stimulation
|
| Organism |
Mus musculus |
| Characteristics |
strain background: 129/SvEv
genotype/variation: STING knockout
cell type: bone marrow derived dendritic cells (BMDCs)
stimulated with: purified tumor-cell derived DNA
|
| Treatment protocol |
BMDCs were stimulated with tumor-derived DNA using Lipofectamin for 7 hours.
|
| Growth protocol |
Bone marrow derived dendritic cells were generated by culturing bone marrow cells in the presence of GM-CSF for 9-12 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Rneasy kit (Qiagen) using manufacture`s instruction.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix Gene Chip Scanner 3000 7G and CEL.
|
| Description |
W3-STING tDNA
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) and dChip software using Affymetrix default analysis settings and global scaling as normalization method. The genes scored as "absent" or with signal intensity <100 were first filtered out.
|
| |
|
| Submission date |
Sep 29, 2014 |
| Last update date |
Sep 30, 2014 |
| Contact name |
Seng-Ryong Woo |
| E-mail(s) |
[email protected]
|
| Phone |
773-702-1263
|
| Organization name |
University of Chicago
|
| Department |
Pathology
|
| Lab |
Dr. Gajewski
|
| Street address |
929 E 57th street
|
| City |
Chicago |
| State/province |
Illinois |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE61835 |
Gene expression by cyotosolic DNA stimulation |
|
