TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
Label
Biotin
Label protocol
cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
Hybridization protocol
Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
Scan protocol
Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
Description
Age at diagnosis: 56, Histology: IDC, Tumor stage: 4, Tumor grade: 3
Data processing
The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
