|
| Status |
Public on Mar 10, 2007 |
| Title |
733T |
| Sample type |
RNA |
| |
|
| Source name |
Mammary gland from patient with human breast cancer
|
| Organism |
Homo sapiens |
| Characteristics |
Human breast cancer
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction of total RNA was performed according to the manufacturer's instructions (Life Technologies).
|
| Label |
Biotin
|
| Label protocol |
cRNA was amplified from 1-2 µg of total RNA using the small sample labeling version II protocol (Affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Following fragmentation, the cRNA was hybridized for 16 hr at 45C on GeneChip® Human Genome U133A Array. Washing steps for the chip, staining with streptavidin-phycoerythrin and signal-amplification were performed according to the manufacturer’s instructions.
|
| Scan protocol |
Each hybridized Affymetrix GeneChip array was scanned with GeneChip Scanner 2500.
|
| Description |
Age at diagnosis: 49, Histology: IDC + ILC, Tumor stage: 1, Tumor grade: 2
|
| Data processing |
The raw experimental microarray data were normalized with the Affymetrix GeneChip analysis software (Microarray Suite Version 5.0). Further analyses were performed with the software “CorrXpression”.
|
| |
|
| Submission date |
Dec 21, 2006 |
| Last update date |
Mar 09, 2007 |
| Contact name |
Andreas Klein |
| E-mail(s) |
[email protected]
|
| Phone |
+49 30 450 528087
|
| Fax |
+49 30 450 52945
|
| URL |
http://www.charite.de/molbiol/bioinf/tumbiol/
|
| Organization name |
Charité (CCO)
|
| Department |
Institute of Biochemistry
|
| Street address |
Charitéplatz 1
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (2) |
| GSE6596 |
Comparison of gene expression data from human and mouse breast cancers: Identification of conserved breast tumor genes |
| GSE6772 |
Comparison of gene expression data from human and mouse breast cancers |
|
