|
| Status |
Public on May 01, 2015 |
| Title |
shCtrl 13-4 |
| Sample type |
RNA |
| |
|
| Source name |
shCtrl
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: MDA-MB231 cell xenograft tumor
treatment: shCtrl
|
| Growth protocol |
Xenograft tumors were generated by injecting a total of 1 x 10^6 MDA-MB231 cells mixed with Matrigel into the mammary fat pad in ICR/SCID mice at 4 wk of age. Tumors were collected after 8 wks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from xenografts using RNeasy Mini Kit (Qiagen)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA (GeneChip® WT Terminal Labeling and Hybridization User Manual for use with the Ambion® WT Expression Kit, 2012, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5.5 ug of cRNA were hybridized for 16 hours at 45°C on HuGene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 7G.
|
| Description |
RMA expression value derived from Expression Console software
|
| Data processing |
The data were analyzed with gene level RMA sketch default analysis settings as normalization method using Affymetrix Expression Console.
|
| |
|
| Submission date |
Oct 18, 2014 |
| Last update date |
May 01, 2015 |
| Contact name |
Keli Xu |
| E-mail(s) |
[email protected]
|
| Phone |
601-815-3083
|
| Organization name |
University of Mississippi Medical Center
|
| Department |
Cancer Institute
|
| Lab |
Keli Xu
|
| Street address |
2500 N. State St.
|
| City |
Jackson |
| State/province |
MS |
| ZIP/Postal code |
39216 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE62481 |
The effect of MFNG knockdown on gene expression profile of xenograft tumor derived from MDA-MB231 cells |
|
