|
| Status |
Public on Oct 23, 2014 |
| Title |
Dental pulp cells control sample triplicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
dental pulp cells obtained from milk teeth
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: dental pulp cells
|
| Treatment protocol |
no treatment protocol was used
|
| Growth protocol |
Dental pulp cells were grown in DMEM/F12 media supplemented with 1% non-essential aminoacids and 15% fetal bovine serum. iPSC cells were grown in mTeSR media (Stem cell Technologies). Embryonic cells were grown in human embryonic stem cells media (DMEM/F12 media supplemented with 20% knockout serum replacement; 50uM b-mercaptoethanol, 1% non-essential aminoacids; 10ng/ml FGF)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using Tryzol based extraction method
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
GeneChip Affymetrix Human Gene 1.0 ST Array
|
| Scan protocol |
GeneChips were scanned using the Scanner 3000 7G System
|
| Description |
gene expression data from dental pulp cells
|
| Data processing |
The data were analyzed using R/Bioconductor affy package
|
| |
|
| Submission date |
Oct 22, 2014 |
| Last update date |
Oct 23, 2014 |
| Contact name |
Alysson Renato Muotri |
| E-mail(s) |
[email protected]
|
| Organization name |
University of California San Diego
|
| Department |
Dept. Cellular & Molecular Medicine
|
| Lab |
Stem Cell Program
|
| Street address |
Scenic Road - Sanford Consortium, Room 3005
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0695 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE62622 |
Expression study of human embryonic stem cells, dental pulp cells (DPCs) and induced pluripotent stem cells (iPSC) obtained from DPC for characterization of iPSC |
| GSE62632 |
Modeling non-syndromic autism and the impact of TRPC6 disruption in human neurons |
|
