|
| Status |
Public on Jun 17, 2015 |
| Title |
DMSO_Rep1 |
| Sample type |
RNA |
| |
|
| Source name |
DMSO treated ARPE-19 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: ARPE-19
agent: DMSO
|
| Treatment protocol |
ARPE-19 cells were treated with SRPIN803 (10 µM) or the control (0.1% DMSO) for 4 hours.
|
| Growth protocol |
ARPE-19 cells were maintained at 37 ºC in a 5% CO2-air incubator with Dulbecco's modified Eagle's medium (DMEM)/Ham's F-12 supplemented with 10% fetal bovine serum and 1% Penicillin‐Streptomycin Mixed Solution
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Sepasol®-RNA I Super G (nacalai tesque) according to the manufacturer's instruction. The Isolated total RNA was treated with RQ1 DNase (QIAGEN) and was purified with Sepasol®-RNA I Super G again.
|
| Label |
biotin
|
| Label protocol |
Biotinylated aRNA were prepared from 100 ng total RNA using GeneChip® 3' IVT Express Kit (Affymetrix) from 100 ng total RNA according to the manufacturer's instruction
|
| |
|
| Hybridization protocol |
Following fragmentation, 12 ug of aRNA were hybridized for 16 hr at 45C on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained using GeneChip Hybridization Wash and Stain Kit in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000G
|
| Description |
Gene expression data from DMSO treated ARPE-19 cells
|
| Data processing |
The data were analyzed with RMA using Agilent GeneSpringGX 12.6.1 software.
|
| |
|
| Submission date |
Nov 04, 2014 |
| Last update date |
Jun 17, 2015 |
| Contact name |
Masatsugu Denawa |
| Organization name |
Kyoto University
|
| Street address |
Yoshida-Konoe-cho, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
6068501 |
| Country |
Japan |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE62947 |
Expression Data from DMSO or SRPIN803 treated ARPE-19 cells |
|
