|
| Status |
Public on Feb 09, 2017 |
| Title |
CHC001 PDX NT - replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
CHC001 PDX NT - replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
xenograft source: patient-derived CHC001 PDX
treatment: PBS
|
| Treatment protocol |
EGI-1 xenografts and CHC001 PDXs were treated with 0.15 mg/Kg weekly i.v. (three administrations) or with PBS alone
|
| Growth protocol |
RPMI + 10%FBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. Absolutely RNA miRNA Kit
|
| Label |
Cy3
|
| Label protocol |
100 ng of mRNA was amplified and labeled using One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labeling kit (Agilent)
|
| |
|
| Hybridization protocol |
600 ng of labeled samples were hybridized on 8 x 60K glass array; after 17 hours of incubation at 65°C, the slide was washed following the manufacturers' instructions.
|
| Scan protocol |
Images were quantified using Agilent Feature Extraction Software (version 9.5).
|
| Data processing |
Raw data were background corrected (normexp method) and quantile normalized using bioconductor.
|
| |
|
| Submission date |
Nov 06, 2014 |
| Last update date |
Apr 23, 2018 |
| Contact name |
Paola Ostano |
| Organization name |
Fondazione Edo ed Elvo Tempia
|
| Street address |
via Malta 3
|
| City |
Biella |
| ZIP/Postal code |
13900 |
| Country |
Italy |
| |
|
| Platform ID |
GPL17077 |
| Series (1) |
| GSE63043 |
Anti-cancer effect and gene modulation of ET-743 in human biliary tract carcinoma preclinical models |
|
| Relations |
| Reanalyzed by |
GSE113533 |
