|
| Status |
Public on May 01, 2007 |
| Title |
ahg3-1 rep1 |
| Sample type |
RNA |
| |
|
| Source name |
ABA hypersensitive mutant ahg3-1 seedlings
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ABA hypersensitive mutant ahg3-1 columbia back ground
|
| Treatment protocol |
After stratification at 4°C for 4 days, seeds were allowed to germinate on the MS-2% sucrose plate containing 0.5 uM ABA for two days
|
| Growth protocol |
The plates were kept in a growth chamber with a conditions; 23°C, 16 hr light and 8 hr dark cycles
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from frozen tissue using TRIzol reagent (Invitrogen) and purified using a RNease MinElute Cleanup Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
First-strand of cDNA was synthesized using 16 µg of total RNA and SuperScript Double-stranded cDNA Synthesis kit (Invitrogen). The labeled cRNA was synthesized using BioArray RNA Transcripts Labeling Kit (T7) (ENZO Life Sciences inc.). After labeling, the cRNA was digested partially by incubating in 40 mM Tris-acetate, pH8.0, 100 mM KOAc, 30 mM MgOAc, at 94°C for 35 min. Then 30 µg cRNA was used for microarrary hybridization.
|
| |
|
| Hybridization protocol |
30 ug cRNA was used. ATH1 GeneChip was used.
|
| Scan protocol |
Using GSC3000 scannar according to the manufacturer’s recommendation
|
| Description |
Gene expression data of wild type geminating seedlings undernomal condition
|
| Data processing |
RMA normalized using Cel file with affllmGUI
|
| |
|
| Submission date |
Jan 04, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Takashi Hirayama |
| E-mail(s) |
[email protected]
|
| Fax |
+81-86-434-1213
|
| Organization name |
Okayama University
|
| Department |
Institute of Plant Science and Resources
|
| Lab |
Environmental Response Systems
|
| Street address |
2-20-1 Chuo
|
| City |
Kurashiki |
| State/province |
Okayama |
| ZIP/Postal code |
710-0046 |
| Country |
Japan |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE6638 |
Expression data of germinating ahg1, ahg3 and WT seedling in the presence of ABA |
|
| Relations |
| Reanalyzed by |
GSE119083 |
