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Sample GSM1550 Query DataSets for GSM1550
Status Public on May 01, 2002
Title BY-2.7.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) [email protected]
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1380 137 1287 108 0.064736 0
1 809 52 983 102 -0.230375 0
2 863 52 40328 120 0
3 756 52 965 117 -0.280015 0
4 281 52 365 94 -0.254471 0
5 901 56 820 92 0.203487 0
6 587 56 755 96 -0.323093 0
7 1249 55 1347 91 -0.084560 0
8 1382 56 2304 90 -0.751101 0
9 543 54 523 87 0.153980 0
10 1195 53 996 87 0.317684 0
11 7206 55 5842 90 0.302555 0
12 4380 56 1314 88 1.806881 0
13 2141 56 2102 86 0.037026 0
14 601 55 440 86 0.613626 0
15 3624 61 2867 94 0.350119 0
16 3974 63 3425 93 0.219623 0
17 2158 66 1820 96 0.267597 0
18 1389 67 1568 97 -0.165601 0
19 14310 61 8253 91 0.792340 0

Total number of rows: 7085

Table truncated, full table size 210 Kbytes.




Supplementary data files not provided
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