|
| Status |
Public on Sep 28, 2007 |
| Title |
24h_Estradiol_1 |
| Sample type |
RNA |
| |
|
| Source name |
MCF-7
|
| Organism |
Homo sapiens |
| Characteristics |
human breast adenocarcinoma epithelial cell line, ER+, derived from pleural effusion of a 69year-old caucasian woman
|
| Biomaterial provider |
ATCC
|
| Treatment protocol |
MCF-7 were incubated for 24h with extracts/compounds in DMEM containing 10% hormone-free dextran-charcoal stripped FCS
|
| Growth protocol |
cells were incubated in DMEM + 10% FCS for 24h before treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using RNeasy Mini Kit (QIAGEN) according to the manufacturers protocol. RNA quality was checked by UV spectroscopy and agarose (1.5 %) gel electrophoresis (EtBR staining)
|
| Label |
biotin, phycoerythrin-conjugated streptavidin
|
| Label protocol |
Standard Protocol: Affymetrix GeneChip expression Analysis Technical Manual, Version 701021, Rev. 4
|
| |
|
| Hybridization protocol |
Standard Protocol: Affymetrix GeneChip expression Analysis Technical Manual, Version 701021, Rev. 4
|
| Scan protocol |
Standard Protocol: Affymetrix GeneChip expression Analysis Technical Manual, Version 701021, Rev. 4.
Scanning was done using a GeneChip Scanner 3000 7G with default parameters.
|
| Description |
Analysis of gene expression in MCF-7 cells after 24h stimulation with 17beta-Estradiol.
|
| Data processing |
Initial data analysis was performed with Affymetrix MAS5.1 software, setting the scaling of all probe sets to a constant value of 500. Data were further normalized using RMAExpress sofware (release 0.4 alpha 7).
|
| |
|
| Submission date |
Jan 12, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Friedemann Gaube |
| E-mail(s) |
[email protected]
|
| Phone |
+49 3641 949844
|
| Fax |
+49 3641 949842
|
| URL |
http://www.uni-jena.de/Pharmazeutische_Biologie.html
|
| Organization name |
Friedrich-Schiller-University Jena
|
| Department |
Pharmacy
|
| Lab |
Pharmaceutical Biology
|
| Street address |
Semmelweisstr. 10
|
| City |
Jena |
| ZIP/Postal code |
07743 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE6800 |
Effects of Cimicfuga racemosa (black cohosh) in MCF-7 cells |
| GSE6803 |
Effects of Leuzea carthamoides (maral root) in MCF-7 cells |
|
| Relations |
| Reanalyzed by |
GSE64985 |
| Reanalyzed by |
GSE119087 |
