|
| Status |
Public on May 17, 2007 |
| Title |
cells grown with 0% oxygen + 100mM nitrate #1 |
| Sample type |
RNA |
| |
|
| Source name |
strain PAO1 grown with nitrate #1
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
Pseudomonas aeruginosa strain PAO1 grown with nitrate #1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Pseudomonas aeruginosa cells were disrupted by sonication and RNA was purified with the RNeasy Mini kit (Qiagen) including DNase treatment on the column.
|
| Label |
Biotinylated GeneChip reagent
|
| Label protocol |
As recommended by the manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
As recommended by the manufacturer (Affymetrix)
|
| Scan protocol |
As recommended by the manufacturer (Affymetrix)
|
| Description |
cDNA synthesis, fragmentation, labeling, hybridization, and processing of Pseudomonas aeruginosa GeneChips were carried out as recommended by the manufacturer. Experiments were performed in duplicate with independently prepared samples.
|
| Data processing |
The Affymetrix GeneChip Operating Software Version 1.4 was used for initial data acquisition and processing.
|
| |
|
| Submission date |
Jan 12, 2007 |
| Last update date |
May 17, 2007 |
| Contact name |
Carolina Alvarez |
| E-mail(s) |
[email protected]
|
| Phone |
206-221-2798
|
| Organization name |
University of Washington
|
| Department |
Microbiology
|
| Lab |
Harwood
|
| Street address |
1959 NE Pacific St
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE6741 |
Responses of Pseudomonas aeruginosa to low oxygen |
|
