|
| Status |
Public on Mar 06, 2015 |
| Title |
Data 203 Area 4 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
GC_Atretique
|
| Organism |
Bos taurus |
| Characteristics |
breed (cattle): Holstein
tissue: ovary
follicular size: > 9 mm
cell collection method: dissection
follicular status: atretic phase
cell type: granulosa cell
|
| Treatment protocol |
Flow cytometry (FlowJo software) was used to classifiy the follicles among the three growth stages (growing, plateau and atresia).
|
| Growth protocol |
Dominant follicles were collected from slaughterhouse ovaries. Diameter was measured on ovary surface and follicles >9mm were collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using PicoPure RNA Isolation kit (Life Technologies Inc., Burlington, ON).
[amp protocol] Linear amplification was done using RiboAmp HSPlus RNA Amplification Kit (Life Technologies Inc., Burlington, ON) .
|
| Label |
Cy5
|
| Label protocol |
825 ng of total RNA from each sample was labelled with ULS Fluorescent Labelling Kit for Agilent arrays (Kreatech Inc., Durham, NC).
|
| |
|
| Channel 2 |
| Source name |
GC_Croissant
|
| Organism |
Bos taurus |
| Characteristics |
breed (cattle): Holstein
tissue: ovary
follicular size: > 9 mm
cell collection method: dissection
follicular status: growing phase
cell type: granulosa cell
|
| Treatment protocol |
Flow cytometry (FlowJo software) was used to classifiy the follicles among the three growth stages (growing, plateau and atresia).
|
| Growth protocol |
Dominant follicles were collected from slaughterhouse ovaries. Diameter was measured on ovary surface and follicles >9mm were collected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using PicoPure RNA Isolation kit (Life Technologies Inc., Burlington, ON).
[amp protocol] Linear amplification was done using RiboAmp HSPlus RNA Amplification Kit (Life Technologies Inc., Burlington, ON) .
|
| Label |
Cy3
|
| Label protocol |
825 ng of total RNA from each sample was labelled with ULS Fluorescent Labelling Kit for Agilent arrays (Kreatech Inc., Durham, NC).
|
| |
|
| |
| Hybridization protocol |
Samples were prepared with Gene Expression Hybridization Kit (Agilent, Santa Clara, CA) then hybridized onto Agilent-manufactured EmbryoGENE bovine microarray slides, using Agilent hybridization chambers.
|
| Scan protocol |
Microarray slides were read by the Tecan’s PowerScanner with the Autogain procedure on each individual array.
|
| Data processing |
Images were processed with Array-Pro Analyzer 6.4 (Media Cybernetics, Rockville, MD) and the FlexArray software (1.6.1) was used for normalization and correction of the background.
|
| |
|
| Submission date |
Dec 05, 2014 |
| Last update date |
Mar 06, 2015 |
| Contact name |
Annie Girard |
| Organization name |
Université Laval
|
| Department |
Sciences animales
|
| Street address |
2440, boul. Hochelaga
|
| City |
Quebec |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 0A6 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13226 |
| Series (1) |
| GSE63918 |
Bovine granulosa cells of dominant follicles; comparison of growing vs atretic |
|
