|
| Status |
Public on Mar 01, 2015 |
| Title |
Newman_rep3 |
| Sample type |
SRA |
| |
|
| Source name |
RNA cell extract
|
| Organism |
Staphylococcus aureus |
| Characteristics |
strain: Newman
|
| Growth protocol |
exponential phase of growth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lyzed with glass beads in a Fast Prep instrument and RNAs extract with Phenol/Chloroform. Ribosomal RNAs were depleted using the Ribo-zero rRNA remmoval kit (bacteria).
NEBNext ultra directional RNA library prep kit (E7420)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1500 |
| |
|
| Description |
ribosomal-depleted RNA
|
| Data processing |
Sequenced reads were verified by a fastqc report
reads were mapped using the BWA aligner
Reads were filtered for reads properly paired and sorted by pairs
Read pairs were counted using HTSeq count
Genome_build: Staphylococcus aureus subps, aureus N315 or Newman
Supplementary_files_format_and_content: file includes raw counts
|
| |
|
| Submission date |
Dec 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Yoann AUGAGNEUR |
| E-mail(s) |
[email protected]
|
| Organization name |
University Rennes 1
|
| Department |
Pharmaceutical Biochemistry
|
| Lab |
INSERM U835
|
| Street address |
2 Avenue du Pr Leon Bernard
|
| City |
RENNES |
| ZIP/Postal code |
35043 |
| Country |
France |
| |
|
| Platform ID |
GPL19531 |
| Series (1) |
| GSE64026 |
SRD: a Staphylococcus Regulatory RNA Database |
|
| Relations |
| BioSample |
SAMN03257083 |
| SRA |
SRX803163 |
