|
| Status |
Public on Dec 16, 2014 |
| Title |
AE104 with 50 µM EDTA, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
cells of C. metallidurans
|
| Organism |
Cupriavidus metallidurans CH34 |
| Characteristics |
genotype/variation: AE104
treatment: 50 µM EDTA
|
| Growth protocol |
C. metallidurans strains were grown in Mineral salts medium + 0.2 % Na-gluconate with or without ZnCl2 or EDTA to a cell turbidity of 100 Klett, then cells were harvest by centrifugation for 1' at 4°C in 2.0 ml Tubes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen) was used according to the manufacturers manual including on-column DNase digestion. PCR were done to check for DNA contaminations, only RNA was used that did not generate products in the PCR reaction. RNA quality was checked on formamide gels and measured as RIN on Agilent 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany).
|
| Label |
Cy3
|
| Label protocol |
Labeling was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| |
|
| Hybridization protocol |
Hybridisation was done by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Scan protocol |
Agilent DNA microarray Scanner were used by the service provider IMGM Laboratories GmbH (Martinsried)
|
| Data processing |
First, dark and light control spots, and foreign DNA control spots were deleted. Secondly, if genes were represented by more than one spot, the mean value coming from these two, three or more spots was used and assigned to the gene number. The mean intensity of the pixels of the surrounding area was subtracted from the mean density of the pixels of the spots to give the signal strength
|
| |
|
| Submission date |
Dec 15, 2014 |
| Last update date |
Jan 07, 2015 |
| Contact name |
Cornelia Große |
| E-mail(s) |
[email protected]
|
| Organization name |
Martin-Luther-University Halle-Wittenberg
|
| Department |
Faculty of Biology/Microbiology
|
| Street address |
Kurt-Mothes-Str.3
|
| City |
Halle/Saale |
| ZIP/Postal code |
06120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16115 |
| Series (1) |
| GSE64196 |
Stress response of Cupriavidus metallidurans strains |
|
