|
| Status |
Public on Jun 05, 2007 |
| Title |
Rb null fetal liver 2, embryo at 12.5 days of development |
| Sample type |
RNA |
| |
|
| Source name |
Rb heterozygous 129sv matings
|
| Organism |
Mus musculus |
| Characteristics |
genotype:Rb null
Age: e12.5
|
| Treatment protocol |
untreated embryos
fetal liver dissected and disaggregated in PBS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from e12.5 mouse fetal liver
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 18, 2007 |
| Last update date |
Jun 05, 2007 |
| Contact name |
Kay F Macleod |
| E-mail(s) |
[email protected]
|
| Organization name |
Ben May Institute/University of Chicago
|
| Street address |
927 E 57th St
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE6787 |
Expression data from wildtype and Rb-/- fetal liver at e12.5 |
|
