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Sample GSM1578 Query DataSets for GSM1578
Status Public on May 01, 2002
Title BY-3.3.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) [email protected]
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 367 80 408 104 -0.238353 0
1 244 80 299 103 -0.412491 0
2 222 80 308 100 -0.706025 0
3 226 83 317 98 -0.770248 0
4 163 84 222 96 -0.828832 0
5 319 83 309 95 -0.014157 0
6 555 96 511 100 0.004023 0
7 348 88 397 93 -0.380892 0
8 337 83 397 91 -0.424036 0
9 293 83 301 92 -0.148446 0
10 402 84 382 91 -0.027325 0
11 2025 87 1520 92 0.285240 0
12 1203 87 1781 92 -0.753165 0
13 548 86 540 91 -0.114155 0
14 251 86 250 90 -0.110939 0
15 796 87 792 91 -0.138962 0
16 1165 88 1003 93 0.087747 0
17 1011 88 844 91 0.138348 0
18 525 91 440 95 0.175766 0
19 11338 96 7059 96 0.535785 0

Total number of rows: 7085

Table truncated, full table size 210 Kbytes.




Supplementary data files not provided
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