|
| Status |
Public on Jan 17, 2015 |
| Title |
Embryonic day 18 replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mouse liver from embryonic day 18
|
| Organism |
Mus musculus |
| Characteristics |
Stage: Embryonic
age: embryonic day 18
cell type: immature hepatocytes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from whole livers taken at different developmental timepoints (embryonic day 14, embryonic day 18, post-natal day 5 and post-natal day 56) along with hepatoblasts isolated from E14 livers and immature hepatocytes isolated from E18 livers was extracted and purified using the Qiagen RNeasy Mini Kit.
|
| Label |
Cy5
|
| Label protocol |
2 µg of total RNA was reverse transcribed and amplified by using an RNA amplification kit from Ambion. Fifteen micrograms of amplified RNA were labeled by direct chemical coupling to the Cy5 NHS ester (Amersham Biosciences). Normal adult mouse liver (Agilent) was used as control and Cy3 labeled. Labeled RNAs were purified, fragmented, and used as probes to hybridize microarrays.
|
| |
|
| Channel 2 |
| Source name |
Normal adult mouse liver
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from whole livers taken at different developmental timepoints (embryonic day 14, embryonic day 18, post-natal day 5 and post-natal day 56) along with hepatoblasts isolated from E14 livers and immature hepatocytes isolated from E18 livers was extracted and purified using the Qiagen RNeasy Mini Kit.
|
| Label |
Cy3
|
| Label protocol |
2 µg of total RNA was reverse transcribed and amplified by using an RNA amplification kit from Ambion. Fifteen micrograms of amplified RNA were labeled by direct chemical coupling to the Cy5 NHS ester (Amersham Biosciences). Normal adult mouse liver (Agilent) was used as control and Cy3 labeled. Labeled RNAs were purified, fragmented, and used as probes to hybridize microarrays.
|
| |
|
| |
| Hybridization protocol |
Hybridization procedure followed manufacturer direction
|
| Scan protocol |
Scanned on Axon Instruments (V1.00) GenePix 4000B scanner
|
| Description |
Biological replicate 3 of 3.
|
| Data processing |
Microarray data for liver samples used in this study was normalized and analyzed with limma R package
|
| |
|
| Submission date |
Jan 16, 2015 |
| Last update date |
Jan 17, 2015 |
| Contact name |
Jinyu Li |
| E-mail(s) |
[email protected]
|
| Organization name |
Stony Brook University
|
| Department |
Cancer Center
|
| Street address |
101 Nicolls Rd
|
| City |
Stony Brook |
| State/province |
NY |
| ZIP/Postal code |
11794 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE65063 |
Reciprocal interaction of Wnt and RXR-α pathways in hepatocyte development and hepatocellular carcinoma |
|
