|
| Status |
Public on Aug 03, 2007 |
| Title |
WT30 |
| Sample type |
RNA |
| |
|
| Source name |
B6, HFD 2wks
|
| Organism |
Mus musculus |
| Characteristics |
B6 wild type
Age: 4-8 wks
|
| Treatment protocol |
Mice were feed high-fat diet(TD88137) for 2 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction and Qiagen Column.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Genome Array.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from wt B6 mice liver HFD 2 weeks.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Jan 29, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Yugang Wang |
| E-mail(s) |
[email protected]
|
| Phone |
773-702-0928
|
| Fax |
773-834-8940
|
| Organization name |
University of Chicago
|
| Department |
Pathology
|
| Street address |
5841 S. Maryland
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6903 |
Expression data from high-fat diet feeded WT and LIGHT Tg mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
