|
| Status |
Public on Aug 30, 2007 |
| Title |
Placenta Adra2bWT 3 |
| Sample type |
RNA |
| |
|
| Source name |
placenta (E 10.5)
|
| Organism |
Mus musculus |
| Characteristics |
The placenta (E 10.5) of a congenic adra2B+/+ mouse embryo (maternal genotype Adra2b+/-) was rapidly dissected and flash frozen.
|
| Biomaterial provider |
Institute of Pharmacology, University of Freiburg
|
| Treatment protocol |
No additional treatment was applied.
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA (5 µg) from Placenta (E10.5) was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany).
|
| Label |
biotin
|
| Label protocol |
Double stranded cDNA was synthesized (GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents, 900493) and biotin labeled cRNA was in vitro transcribed (GeneChip® Expression 3' Amplification One-Cycle Target Labeling and Control Reagents, 900493). Cleanup of the cDNA and the fragmented cRNA was carried out with the GeneChip Sample Module (Affymetrix,900371). The integrity of the total RNA and of the subsequent preparation steps was validated using the Agilent 2100 bioanalyzer (Waldbronn, Germany).
|
| |
|
| Hybridization protocol |
10 µg of fragmented cRNA were hybridized (16h) to the array. Staining and washing was conducted according to the Affymetrix protocol.
|
| Scan protocol |
The processed array was scanned using a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
The whole procedure was carried out according to the Affymetrix standard protocol (Eukaryotic Sample and Array Processing).
|
| Data processing |
Data was analyzed using ArrayAssist 5.0 software (Stratagene). Probe levels were calculated using the GCRMA algorithm.
|
| |
|
| Submission date |
Jan 30, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ralf Gilsbach |
| E-mail(s) |
[email protected]
|
| Organization name |
Institute of Pharmacology
|
| Lab |
Prof. Dr. Lutz Hein
|
| Street address |
Albertstr. 25
|
| City |
Freiburg |
| ZIP/Postal code |
79104 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE6909 |
Differential gene expression in placentae (E10.5) from adra2bKO and adra2bWT mice |
|
| Relations |
| Reanalyzed by |
GSE119085 |
