|
| Status |
Public on Oct 01, 2015 |
| Title |
GFP-positive cells from Df(1)CHES-1-like1; twi-GAL4 UAS-2EGFP embryos, rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
FACS-sorted Drosophila cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell type: FACS-sorted GFP positive cells
age: cell suspensions of stage 11/early stage 12 embryos
genotype/variation: Df(1)CHES-1-like1; twi-GAL4 UAS-2EGFP
|
| Growth protocol |
Embryos were aged to stage 11-early stage 12 at 25°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions of sorted mesodermal cells.
|
| Label |
biotin
|
| Label protocol |
Approximately 2-5 µg of total RNA was processed to produce biotinylated cRNA targets
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
FACS-sorted GFP positive cells from single cell suspensions of stage 11/early stage 12 embryos which are homozygous for the CHES-1-like null mutation Df(1)CHES-1-like1 and possess both twi-GAL4 and UAS-2EGFP insertions that target GFP fluorescence to Twist expressing mesodermal cells
|
| Data processing |
The data were analyzed with the Bioconductor affy package, using the rma command with default settings, and log2 transformed.
|
| |
|
| Submission date |
Jan 29, 2015 |
| Last update date |
Oct 01, 2015 |
| Contact name |
Shaad M. Ahmad |
| E-mail(s) |
[email protected]
|
| Organization name |
National Heart Lung and Blood Institute
|
| Department |
Systems Biology Center
|
| Lab |
Laboratory of Developmental Systems Biology
|
| Street address |
Building 10, Room 7N311, 10 Center Drive MSC 1654
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL1322 |
| Series (1) |
| GSE65439 |
Two Forkhead transcription factors regulate cardiac progenitor specification by controlling the expression of receptors of the fibroblast growth factor and Wnt signaling pathways |
|
