Array Type: Rat230_2 Amount to Core: 15 Units of Amount: ul OD260: 1.675 OD280: 0.838 Ratio: 1.96 Dilution: 1:5 RNA Integrity Number: 8.6 Bioanalyzer Results: Good Sample Preservation: Cells resuspended in RNAlater and stored at 4 degrees Centigrade Strain or Line: F344/NCrl NCBI Taxonomy ID: 10116 Individual: 234 Developmental State: Adult Sacrifice Method: Deep pentobarbital anesthesia followed by exsanguination Sex: Male Units of Age: weeks
Biomaterial provider
Ed Bermudez, CIIT Centers for Health Research, 6 Davis Drive, PO Box 12137, Research Triangle Park, NC, 27709, Russell Thomas, [email protected], 919 558 1311, 919 558 1300
Treatment protocol
in vivo Treatment: Formaldehyde, Treatment Repeat: 5, Units of Treatment Repeat: days per week, Treatment Duration: 19, Units of Duration: days, Treatment Time: 6, Units of Treatment Time: hrs per day, Route of Exposure: Inhalation (whole-body), Dose or Concentration: 2, Units of Dose or Concentration: ppm
Extracted molecule
total RNA
Extraction protocol
Extraction Method: Qiagen Micro RNA kit
Label
biotin
Label protocol
standard Affymetrix protocol
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Sample Type: Cells, Tissue: nasal epithelial cells (level II region), Tissue Harvest Methods: The nose was dissected to isolate the Level II region of both nostrils. The maxillo turbinate and olfactory epithelium were discarded and the lateral meatus and nasoturbinate were retained for cell isolation. The dissected nasal sections were rinsed with cold phosphate buffered saline to remove the blood. After rinsing, a 5 ml buffered mixture of proteases (collagenase, 80 U/ml, Sigma-Aldrich; pronase, 10 mg/ml, Sigma-Aldrich; HEPES, 0.1 M) in Hams F12 medium (Invitrogen, Carlsbad, CA) was added to the nose and incubated at 37°C for 40 minutes to remove the epithelial cell layer. Following incubation, the mixture was vortexed and the resulting cell suspension was collected in a clean centrifuge tube. The cell suspension was centrifuged (200 x g, 10 min, 10°C) and the cell pellet was resuspended in 50 ?L PBS and 200 ?L RNAlater (Ambion, Austin, TX). The epithelial cells acquired consisted primarily of transitional epithelium with some respiratory epithelium., Harvest Time Point: 19, Units of Harvest Time Point: days nasal epithelium, formaldehyde inhalation exposure, 2 ppm, 6 hours per day, 5 days per week, 19 day time point
Data processing
affylmGUI, The Walter and Eliza Hall Institute of Medical Research
