|
| Status |
Public on Feb 04, 2015 |
| Title |
MCL001_dNSP16-MERS_7hr_3_microRNA |
| Sample type |
RNA |
| |
|
| Source name |
Calu-3 cells, dNSP16-MERS treatment, 7 hours, replicate 3
|
| Organism |
Homo sapiens |
| Characteristics |
time: 7 hours
virus: dNSP16-MERS
replicate: 3
|
| Treatment protocol |
Cells were washed twice with PBS and infected with a multiplicity of infection of 5.
|
| Growth protocol |
SCL002.0P: Maintenance, Plating and Virus Infection of Calu-3 cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TCL001.0P: Preparation of Samples from Calu-3 cells for Isolation of RNA.
|
| Label |
Cy3
|
| Label protocol |
Total RNA from each sample was labeled using miRNA Complete Labeling and Hyb Kit (v2.4, Agilent Technologies)
|
| |
|
| Hybridization protocol |
Human miRNA MicroarraySlide (8x60K, Agilent Technologies)
|
| Scan protocol |
Agilent Scanner G2505C
|
| Data processing |
Scanned images were analyzed using Agilent Feature Extraction Software (v11.0.1.1). Quantile normalization and data processing performed using GeneSpring GX v12.0 software (Agilent Technologies).
|
| |
|
| Submission date |
Feb 03, 2015 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Natalie Heller |
| E-mail(s) |
[email protected]
|
| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
|
| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
| |
|
| Platform ID |
GPL19730 |
| Series (2) |
| GSE65574 |
Human Calu-3 cell transcriptome response to a wild type infectious clone of Middle Eastern Respiratory Syndrome (icMERS) coronavirus and icMERS mutant viruses |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
|
