Subconfluent RBP2-/- primary mouse embryonic fibroblasts at passage 3.
Extracted molecule
total RNA
Extraction protocol
Total RNA isolation using RNeasy mini kit with on-column DNase digestion (Qiagen).
Label
Biotin
Label protocol
Affymetrix
Hybridization protocol
Affymetrix
Scan protocol
Affymetrix
Description
Study the effect of RBP2 inactivation in gene expression Analysis name: WK2006060808
Data processing
Analysis of the raw gene expression profiling was performed using dChip software. Please see the following paper for details. Klose RJ, Yan Q, Tothova Z, Yamane K, Erdjument-Bromage H, Tempst P, Gilliland DG, Zhang Y, Kaelin WG. The retinoblastoma binding protein RBP2 is an H3K4 demethylase. Cell (2007)
