|
| Status |
Public on Nov 29, 2007 |
| Title |
Transgenic nr 661 |
| Sample type |
RNA |
| |
|
| Source name |
Mus Musculus Kidney
|
| Organism |
Mus musculus |
| Characteristics |
Genotype: FGF23 TG on C57Bl strain
Age: 8 weeks
|
| Treatment protocol |
Transgenic mice were produced with microinjection technique with construct of human WT FGF23 behind a 1 alpha col 1 promotor
|
| Growth protocol |
Kidneys were collected at 8 weeks of age
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner GA 2500.
|
| Description |
Gene expression data from kidneys of 8 week FGF23 TG mice and WT littermates
|
| Data processing |
Subsequent analysis of the gene expression data was carried out in the statistical computing language R using packages available from the Bioconductor project. The raw data was normalized using the GCRMA method.
|
| |
|
| Submission date |
Feb 07, 2007 |
| Last update date |
Feb 07, 2007 |
| Contact name |
Richard Marsell |
| E-mail(s) |
[email protected]
|
| Phone |
+46186112657
|
| Fax |
+4618500952
|
| Organization name |
Uppsala University
|
| Department |
Dep. of Surgical Sciences
|
| Lab |
Bone lab
|
| Street address |
Sjukhusvägen
|
| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE6979 |
Gene expression analysis of Kidneys from FGF23 TG and WT mice |
|
