|
| Status |
Public on Apr 23, 2007 |
| Title |
Mouse ileum with L.monocytogenes_D_5550 |
| Sample type |
RNA |
| |
|
| Source name |
Fabpi-hEcad transgenic C57BL/6J mouse ileum- 72h monoassociation with L.monocytogenes_D_5550
|
| Organism |
Mus musculus |
| Characteristics |
12-15 week old, male, gnotobiotic Fabpi-hEcad C57BL/6J transgenic mice that express hEcad in small intestinal enterocytes under the control of nucleotides -1178 to +28 of the rat Fabpi gene_ileum (pool of 4 animals)_72h monoassociation with L.monocytogenes_D_5550
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified from ileum of each mouse [Midi RNeasy kit (Qiagen) with on-column DNase digestion]. Equivalent amounts of RNA from mice in each treatment group (n=4/experiment) were pooled and two biotinylated cRNA targets (40 ug/replicate) were independently prepared from each sample.
|
| Label |
biotin
|
| Label protocol |
In vitro transcription (BioArray HighYield RNA Transcript Labeling Kit; Enzo Life Sciences) was performed on cRNA derived from 40ug total RNA followed by standard fragmentation. For detailed protocol see http://pathimm.wustl.edu/~mgacore/protocols.htm
|
| |
|
| Hybridization protocol |
Hybridization performed using standard Affymetrix protocol available at http://www.affymetrix.com/support/technical/manuals.affx
|
| Scan protocol |
Scanning performed using standard Affymetrix protocol available at http://www.affymetrix.com/support/technical/manuals.affx
|
| Description |
ileum (pool of 4 animals)_72h monoassociation with L.monocytogenes_D_5550
|
| Data processing |
MAS5; scaling = 500
|
| |
|
| Submission date |
Feb 10, 2007 |
| Last update date |
Apr 23, 2007 |
| Contact name |
Justin L. Sonnenburg |
| E-mail(s) |
[email protected]
|
| Phone |
314-362-5443
|
| URL |
http://gordonlab.wustl.edu/metaview/bt
|
| Organization name |
Washington University
|
| Department |
Center for Genome Sciences
|
| Lab |
Jeffrey I. Gordon
|
| Street address |
4444 Forest Park
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE7013 |
Gnotobiotic mouse ileum; Listeria infection series |
|
