|
| Status |
Public on Mar 01, 2007 |
| Title |
HeLa cells transfected with UPF1 siRNA 2 (experiment) |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells, 72 hs after transfection
|
| Organism |
Homo sapiens |
| Characteristics |
HeLa cells
(cervical cancer cells)
|
| Extracted molecule |
cytoplasmic RNA |
| Extraction protocol |
protocol used in (Gehring et al., Mol.Cell, 2003); PMID: 12718880
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Oligonucleotide arrays were scanned using a confocal laser scanner (GeneArrayTM, Hewlett Packard, Palo Alto, CA).
|
| Description |
HeLa cells were treated in three independent experiments with UPF1 siRNAs or luciferase siRNAs as a negative control. To positively control for the effect of RNAi the efficient depletion of UPF1 was ascertained by immunoblotting. RNA isolated from these cells was analyzed on Affymetrix HG_U133A GeneChips™. The data here correspond to the second experiment (UPF1 siRNA transfection).
|
| Data processing |
We used the Affymetrix GeneChip Suite 5.0 software (MAS 5.0) to calculate raw expression values for each of the 22,283 probe sets on the U133A oligonucleotide arrays.
|
| |
|
| Submission date |
Feb 12, 2007 |
| Last update date |
Feb 13, 2007 |
| Contact name |
Marcelo Viegas |
| E-mail(s) |
[email protected]
|
| Organization name |
EMBL-MMPU
|
| Lab |
Kulozik/Hentze
|
| Street address |
IM NEUENHEIMER FELD 156
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE7009 |
UPF1-depleted HeLa cells - effect on physiological targets |
|
