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Sample GSM1618 Query DataSets for GSM1618
Status Public on May 01, 2002
Title BY-7.2.c
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) [email protected]
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 3163 67 3440 75 -0.087541 0
1 1949 78 1897 86 0.079683 0
2 1851 79 1261 85 0.624151 0
3 1650 83 2201 90 -0.397261 0
4 897 93 864 101 0.108173 0
5 2601 98 2447 100 0.125501 0
6 697 96 715 97 -50
7 3067 90 3145 94 -0.002763 0
8 3143 87 2847 91 0.181729 0
9 1552 86 1466 91 0.125114 0
10 2719 95 2795 93 -0.009600 0
11 11726 92 9539 95 0.333537 0
12 4885 107 12658 121 -1.359053 0
13 5519 107 5831 125 -0.043658 0
14 1767 93 1794 99 0.014674 0
15 8050 89 9526 98 -0.211342 0
16 9256 88 9118 101 0.056620 0
17 6870 92 6038 102 0.224029 0
18 3588 101 3126 107 0.240576 0
19 13490 104 7216 103 0.944855 0

Total number of rows: 7085

Table truncated, full table size 218 Kbytes.




Supplementary data files not provided
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