|
| Status |
Public on Aug 31, 2016 |
| Title |
primary ccRCC, patient 7 |
| Sample type |
RNA |
| |
|
| Source name |
kidney
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: ccRCC
tumor staging: pT1
grading: 2
metastasis: M0
age: 69
Sex: female
|
| Treatment protocol |
Immediate stabilization of tissue after surgery in RNAlater RNA Stabilization Reagent (Qiagen, Hilden, Germany; Cat.no. 76104)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation according to the instruction using RNeasyMini Kit (Qiagen, Hilden, Germany; Cat.no. 74104)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared and fragmentated according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
cRNA were hybridized on Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained according to the standard Affymetrix protocol
|
| Scan protocol |
After hypridisation the GeneChips were scanned according to the standard Affymetrix protocol
|
| Data processing |
Affymetrix Expression Console Software with default analysis settings were used to generate corresponding MAS5 CHP files. The CHP-files were analysed by Genespring (vers.12.6) software (normalisation: shift to 75 percentile; baseline transformation: median of all samples)
|
| |
|
| Submission date |
Feb 24, 2015 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Klaus Jung |
| Organization name |
University Hospital Charité
|
| Department |
Dept. of Urology
|
| Lab |
Research Division
|
| Street address |
Schumannstr. 20/21
|
| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE66270 |
Genome-wide analysis of gene expression patterns in human kidney cancer [patients without metastasis] |
| GSE66272 |
Genome-wide analysis of gene expression patterns in human kidney cancer |
|
