|
| Status |
Public on May 01, 2007 |
| Title |
UCB-MSC_UCB1_3.5_hr_U133B |
| Sample type |
RNA |
| |
|
| Source name |
human umbilical cord blood-derived mesenchymal stem cell population 1
|
| Organism |
Homo sapiens |
| Characteristics |
human umbilical cord blood-derived mesenchymal stem cell population 1, 3.5 hr after low-serum synchronization
|
| Biomaterial provider |
Dr Biagio Saitta, Coriell Institute for Medical Research, 403 Haddon Ave, Camden, NJ 08103
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells in culture using the RNeasy Mini Kit (Qiagen), according to manufacturer's protocol
|
| Label |
Biotin
|
| Label protocol |
Microarray target was prepared following the Affymetrix protocol. In brief, cDNA was synthesized from total RNA using the SuperScript II double-stranded cDNA synthesis kit (Invitrogen), followed by in vitro transcription, where labeled cRNA was generated (Enzo).
|
| |
|
| Hybridization protocol |
Hybridization was carried out according to Affymetrix protocol.
|
| Scan protocol |
Scan was carried out according to Affymetrix protocol.
|
| Description |
none
|
| Data processing |
RMA method normalization implemented in BioConductor
|
| |
|
| Submission date |
Feb 12, 2007 |
| Last update date |
Feb 16, 2007 |
| Contact name |
Kenro Kusumi |
| E-mail(s) |
[email protected]
|
| Phone |
480-727-8993
|
| Fax |
480-965-6899
|
| URL |
http://sols.asu.edu/faculty/kkusumi.php
|
| Organization name |
Arizona State University
|
| Department |
School of Life Sciences
|
| Street address |
PO Box 874501
|
| City |
Tempe |
| State/province |
AZ |
| ZIP/Postal code |
85287 |
| Country |
USA |
| |
|
| Platform ID |
GPL97 |
| Series (2) |
| GSE7015 |
Identif. of oscillatory genes in somitogenesis from functional genomic analysis of a human mesenchymal stem cell model |
| GSE7054 |
Identification of oscillatory genes in somitogenesis from functional genomic analysis |
|
