NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1624 Query DataSets for GSM1624
Status Public on May 01, 2002
Title BY-7.3.d
Sample type RNA
 
Channel 1
Source name reference pool of BY4716 strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
Channel 2
Source name segregant from BY4716 x wild wine strain
Organism Saccharomyces cerevisiae
Extracted molecule total RNA
 
 
Description RNA from a haploid F1 segregant from a cross between the
lab strain BY4716 (isogenic to S288c) and a wild isolate
strain from a California vineyard collected by R.
Mortimer. The reference sample is RNA from haploid
BY4716. See Brem et al., Science 296(5568):752-755.
Lot batch =
 
Submission date Apr 26, 2002
Last update date May 28, 2005
Contact name Rachel Brem
E-mail(s) [email protected]
Phone 206-667-5659
Fax 206-667-5978
URL http://www.fhcrc.org/labs/kruglyak/index.html
Organization name Fred Hutchinson Cancer Research Institute
Department Human Biology
Lab Kruglyak
Street address 1100 Fairview Ave. N. D4-100
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL118
Series (1)
GSE37 BYxwild_40

Data table header descriptions
ID_REF Spot number from platform GPL118
CH1_MEAN Median signal at 635 nm
CH1_BKD_MEAN Median background signal at 635 nm
CH2_MEAN Median signal at 532 nm
CH2_BKD_MEAN Median background signal at 532 nm
VALUE First compute l = log2 (median 635 - median background 635) - log2 (median 532 - median background 532). Do this for every spot on chip c. Next compute m, the mean of these l values over all spots on chip c. Now subtract m from every l value on chip c to get the final normalized log ratio n. If the SAMPLE has Cy3 in the name, n is the VALUE here; if the SAMPLE has Cy5 in the name, -n is the VALUE.
FLAG GenePix flag; all spots with flag < 0 (defective spot or data) removed

Data table
ID_REF CH1_MEAN CH1_BKD_MEAN CH2_MEAN CH2_BKD_MEAN VALUE FLAG
0 1438 87 1156 55 0.063493 0
1 778 86 566 56 0.208554 0
2 800 86 405 55 0.796849 0
3 615 86 818 55 -0.760136 0
4 415 88 296 58 0.226608 0
5 1056 93 802 56 0.136640 0
6 612 93 461 58 0.133234 0
7 1138 93 826 56 0.208852 0
8 1222 93 1212 56 -0.265816 0
9 819 93 632 56 0.102180 0
10 1121 94 822 56 0.191299 0
11 6481 96 4896 57 0.168257 0
12 3528 97 7397 59 -1.328478 0
13 2197 96 1731 61 0.099507 0
14 709 96 714 60 -0.325124 0
15 3176 95 3110 58 -0.218077 0
16 3804 99 3641 60 -0.182610 0
17 3140 101 2087 62 0.353954 0
18 1519 97 913 59 0.503893 0
19 14078 98 7762 60 0.628339 0

Total number of rows: 7085

Table truncated, full table size 215 Kbytes.




Supplementary data files not provided
| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap