|
| Status |
Public on May 31, 2007 |
| Title |
Primary brown adipocytes 4 days in culture B_y_05 |
| Sample type |
RNA |
| |
|
| Source name |
Primary BAT cells 4 days after isolation
|
| Organism |
Mus musculus |
| Characteristics |
Primary brown adipocytes
4 days in culture
|
| Treatment protocol |
Non differentiated
|
| Growth protocol |
In vitro culture
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix single round amplification protocol from 5 ug total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from primary cell culture
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
|
| |
|
| Submission date |
Feb 14, 2007 |
| Last update date |
Feb 14, 2007 |
| Contact name |
Ola Larsson |
| E-mail(s) |
[email protected]
|
| Phone |
+46 (0)8 517 73280
|
| Organization name |
Karolinska Institutet
|
| Department |
Department of oncology-pathology
|
| Street address |
CCK, R8:01
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE7032 |
Brown and white adipocyte differentiation |
|
