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Sample GSM162541 Query DataSets for GSM162541
Status Public on May 31, 2007
Title Primary brown adipocytes 7 days in culture B_o_22
Sample type RNA
 
Source name Primary BAT cells 7 days after isolation
Organism Mus musculus
Characteristics Primary brown adipocytes
7 days in culture
Treatment protocol Differentiated
Growth protocol In vitro culture
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix single round amplification protocol from 5 ug total RNA
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from primary cell culture
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 150.
 
Submission date Feb 14, 2007
Last update date Feb 14, 2007
Contact name Ola Larsson
E-mail(s) [email protected]
Phone +46 (0)8 517 73280
Organization name Karolinska Institutet
Department Department of oncology-pathology
Street address CCK, R8:01
City Stockholm
ZIP/Postal code 171 76
Country Sweden
 
Platform ID GPL81
Series (1)
GSE7032 Brown and white adipocyte differentiation

Data table header descriptions
ID_REF Identifier
VALUE MAS5-calculated Signal intensity

Data table
ID_REF VALUE
100001_at 1.41
100002_at 11.27
100003_at 11.62
100004_at 57.43
100005_at 267.59
100006_at 21.92
100007_at 188.34
100009_r_at 15.08
100010_at 63.62
100011_at 40.14
100012_at 16.18
100013_at 291.39
100014_at 26.31
100015_at 51
100016_at 222.93
100017_at 69.5
100018_at 32.23
100019_at 39.09
100020_at 223.37
100021_at 3.64

Total number of rows: 12488

Table truncated, full table size 195 Kbytes.




Supplementary file Size Download File type/resource
GSM162541.CEL.gz 2.6 Mb (ftp)(http) CEL

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