|
| Status |
Public on Aug 19, 2016 |
| Title |
ADULT 28 |
| Sample type |
RNA |
| |
|
| Source name |
whole blood RNA
|
| Organism |
Homo sapiens |
| Characteristics |
age class: 45-55
|
| Treatment protocol |
Blood was sampled from antecubital vein of each subject while seated. The subjects were asked to avoid vigorous physical activity in the 24 h before blood sampling and to fasten from the evening meal until the morning, when samples were obtained.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from each of the two blood samples using Tempus Spin RNA Isolation Kit (Applied Biosystems) as specified in the manufacturer's guidelines. To improve the detection sensitivity of transcripts on microarrays globin mRNA was depleted from a portion of each total RNA sample isolated from TEMPUS Blood RNA tubes using the GLOBINclear™-Human kit (Ambion, TX).
|
| Label |
biotin
|
| Label protocol |
The cRNA labeling and hybridizations were performed according to protocols from Affymetrix Inc. (Santa Clara, CA).
|
| |
|
| Hybridization protocol |
The cRNA labeling and hybridizations were performed according to protocols from Affymetrix Inc. (Santa Clara, CA).
|
| Scan protocol |
Affymetrix GeneChip scanner 3000
|
| Description |
gene expression from whole blood collected at rest, morning fasting
|
| Data processing |
RMA of .CEL files using affy Bioconductor library and Brainarray: HuGene10stv1_Hs_ENTREZG_15.1.0 custom CDF. In RMA, PM values have been background adjusted, normalized using quantile normalization, and expression measure calculated using median polish summarization
|
| |
|
| Submission date |
Mar 24, 2015 |
| Last update date |
Aug 20, 2016 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
[email protected]
|
| Phone |
+39-049-827-6108
|
| Organization name |
University of Padova
|
| Department |
Molecular Medicine
|
| Street address |
via U. Bassi 59/b
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
|
| Platform ID |
GPL16522 |
| Series (1) |
| GSE67220 |
AginAging: a portrait from gene expression profile in blood cells: a portrait from gene expression profile in blood cells. |
|
