|
| Status |
Public on Apr 01, 2015 |
| Title |
Airway epithelium, asthma sample 12 |
| Sample type |
RNA |
| |
|
| Source name |
airway epithelium, asthma, Th2 high
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: airway epithelium
age: 24
gender: female
disease state: asthma
th2 group: high
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total epithelial RNA was isolated using the miRNeasy Mini Kit (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
25 ng of RNA was amplified using the NuGen RNA Ovation Amplification Kit.
|
| |
|
| Hybridization protocol |
2.75 µg of single-stranded DNA was hybridized to Affymetrix U133 Plus 2.0 arrays.
|
| Scan protocol |
Arrays were scanned using the Agilent microarray scanner (Agilent) as per manufacturer's protocol.
|
| Description |
SAGE-005
Airway epithelial gene expression data obtained from 3rd-4th generation bronchi at time of research bronchoscopy.
|
| Data processing |
Data underwent RMA normalization and Combat batch correction. Probeset IDs were defined using the Entrez Gene custom CDF v14 available for the Affymetrix U133 Plus 2.0 array available at brainarray.mbni.med.umich.edu.
|
| |
|
| Submission date |
Mar 31, 2015 |
| Last update date |
Apr 01, 2015 |
| Contact name |
Stephanie A Christenson |
| Organization name |
University of California, San Francisco
|
| Department |
Pulmonary and Critical Care
|
| Street address |
513 PARNASSUS AVENUE, HSE1305
|
| City |
San Francisco |
| State/province |
California |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL16311 |
| Series (1) |
| GSE67472 |
Airway epithelial gene expression in asthma versus healthy controls |
|
