|
| Status |
Public on Aug 23, 2017 |
| Title |
Ctl_Lung_explant_rep6 |
| Sample type |
RNA |
| |
|
| Source name |
Lung Tissue, explant, FD, replicate 6
|
| Organism |
Homo sapiens |
| Characteristics |
group: control
gender: Male
age: 27y
race: White
|
| Treatment protocol |
Lung tissue from IPAH patients and failed donor control subjects was preserved in RNAlater before RNA isolation
|
| Growth protocol |
Lung tissue from IPAH patients and failed donor control subjects was obtained during lung transplantation
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with RNeasy and miRNeasy Mini kits (Qiagen) according to the manufacturer’s protocol. RNA concentration, purity and RNA integrity number (RIN) of microarray assay were determined on a Bioanalyzer (Agilent). Only samples with RIN>=7.0 were acceptable for microarray analysis.
|
| Label |
Cy3
|
| Label protocol |
Total RNA (100 ng) was dephosphorylated with 15 units of calf intestine alkaline phosphatase at 37℃ for 30min then end-labeled with pCp-Cy3 (Agilent Technologies) and T4 RNA ligase (Agilent Technologies) at 16°C for 2h.
|
| |
|
| Hybridization protocol |
Hybridization was 20hr at 55°C with 20 RPM rotation in an Agilent G2545A microRNA hybridization oven.
|
| Scan protocol |
Scanning used an Agilent G2505-C scanner controlled by Agilent Scan Control 7.0 software.
|
| Description |
whole lung tissue miRNA profiling from Failed Donor control
|
| Data processing |
GeneView signal intensity files from Agilent Feature Extraction 9.5.3.1 software for miRNA were imported into Partek GS v6.6. The gTotalGeneSignal values were quantile normalized and transformed to log2 notation after negative values were set to 0.001.
|
| |
|
| Submission date |
Apr 06, 2015 |
| Last update date |
Aug 23, 2017 |
| Contact name |
Li Gao |
| Organization name |
Johns Hopkins University School of Medicine
|
| Department |
Clinical Immunology
|
| Street address |
5501 Hopkins Bayview Circle, Rm. 3B.65B
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platform ID |
GPL18402 |
| Series (1) |
| GSE67597 |
Identifying microRNAs targeting WNT/β-catenin pathway in end-stage idiopathic pulmonary arterial hypertension |
|
