|
| Status |
Public on May 25, 2016 |
| Title |
GRDC VS GRC 2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
35S:ORA47-GR +CYC + Dex
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: seedling
genotype: 35S:ORA47-GR
ecotype: Columbia
age: 7 days
|
| Treatment protocol |
P35S:ORA47-GR transgenic plants treated with CYC for 1 h followed by DEX treatment for 2 h.
|
| Growth protocol |
Arabidopsis seeds of each genotype were sterilized with 0.5% bleach and 0.1% Triton X-100, and then plated on the 1/2 Murashige and Skoog (MS) medium with 1% sucrose and agar. Seeds were treated with vernalization in the darkness for 3 days and then transferred to a walk-in growth chamber of 22℃ under a 16 h light / 8 h dark photoperiod.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using REzol C&T (PROTECH) following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
Following manufacturer's instructions of Agilent Quick Amp Labeling Kit
|
| |
|
| Channel 2 |
| Source name |
35S:ORA47-GR +CYC
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: seedlings
genotype: 35S:ORA47-GR
ecotype: Columbia
age: 7 days
|
| Treatment protocol |
P35S:ORA47-GR transgenic plants treated with CYC for 1 h followed by DEX treatment for 2 h.
|
| Growth protocol |
Arabidopsis seeds of each genotype were sterilized with 0.5% bleach and 0.1% Triton X-100, and then plated on the 1/2 Murashige and Skoog (MS) medium with 1% sucrose and agar. Seeds were treated with vernalization in the darkness for 3 days and then transferred to a walk-in growth chamber of 22℃ under a 16 h light / 8 h dark photoperiod.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using REzol C&T (PROTECH) following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
Following manufacturer's instructions of Agilent Quick Amp Labeling Kit
|
| |
|
| |
| Hybridization protocol |
Following manufacturer's instructions of Agilent Two-color Microarray Based Gene Exppression Analysis v4, Agilent microarray hybridization chamber were used.
|
| Scan protocol |
Scanned on an Agilent Microarray scanner.
|
| Description |
Biological replicate 2 of 2. 35S:ORA47-GR treated with 50 μM CYC as control, normal condition.
|
| Data processing |
Agilent Feature Extraction Software (10.5.1.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Apr 09, 2015 |
| Last update date |
May 26, 2016 |
| Contact name |
Hsing-Yu Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
National Taiwan University
|
| Department |
Institute of Plant Biology
|
| Lab |
Tsan-Piao Lin
|
| Street address |
No. 1, Sec. 4, Roosevelt Rd.
|
| City |
Taipei |
| ZIP/Postal code |
10617 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL9020 |
| Series (1) |
| GSE67712 |
ORA47 binding cis-element prediction |
|
