|
| Status |
Public on Apr 01, 2016 |
| Title |
EDL, KO, mouse 83 |
| Sample type |
RNA |
| |
|
| Source name |
EDL_KO
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6N
mouse id: mouse 83
genotype/variation: CaV1.1 delta E29 knockout
sibling: 1
age: 6 months
gender: female
tissue: extensor digitorum longus (EDL) muscle fibres
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy® Fibrous Tissue Mini kit (Qiagen, Cat. No.74704, Venlo, NL).
|
| Label |
biotin
|
| Label protocol |
Biotinylated hybridization targets were generated using the Affymetrix GeneChip WT Expression kit and the Affymetrix GeneChip WT Terminal Labeling kit.
|
| |
|
| Hybridization protocol |
Target samples were hybridized to Human Gene 1.0 ST v1 microarrays according to the manufacturer's protocols in a Affymetrix hybridization oven.
|
| Scan protocol |
Microarrays were scanned in an Affymetrix 3000 scanner.
|
| Description |
2175_032_N1_83_EDL_mG1_AK_131114_(MoGene-1_0-st-v1).CEL.gz
|
| Data processing |
Raw microarray data was pre-processed using the generalgcrma package and our custom transcript-level CEL definition file (CDF) that defines probe sets for each transcript of all genes in the Ensembl database version 75. After GCRMA pre-processing a representative transcript probe set was selected for each gene based on a combination of its average expression and variance of expression across all EDL or Soleus samples.
|
| |
|
| Submission date |
Apr 13, 2015 |
| Last update date |
Apr 01, 2016 |
| Contact name |
Johannes Rainer |
| E-mail(s) |
[email protected]
|
| Organization name |
Eurac Researc
|
| Department |
Institute for Biomedicine
|
| Lab |
Biomedical Informatics
|
| Street address |
Via A. Volta 21
|
| City |
Bolzano |
| ZIP/Postal code |
39100 |
| Country |
Italy |
| |
|
| Platform ID |
GPL20023 |
| Series (1) |
| GSE67803 |
Aberrant calcium influx causes fiber type shift and mitochondrial loss in skeletal muscle |
|
