|
| Status |
Public on Apr 28, 2015 |
| Title |
N |
| Sample type |
RNA |
| |
|
| Source name |
cumulus cells from non-PCOS patients
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
diagnosis: non-polycystic ovary syndrome (non-PCOS)
cell type: cumulus cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated using the mirVana miRNA isolation kit (Ambion, Austin, TX, USA) following manufacturer’s protocol
|
| Label |
biotin
|
| Label protocol |
not provided
|
| |
|
| Hybridization protocol |
not provided
|
| Scan protocol |
The arrays were stained with Fluidics Station 450 using fluidics script FS450_0003 (Affymetrix), and then scanned on an GeneChip microarray scanner (Affymetrix)
|
| Data processing |
miRNA probe outliers were defined as per the manufacturer's instructions (Affymetrix) and further analyzed for data summarization, normalization and quality control by using the miRNA QC Tool software (www.affymetrix.com)
To determine the significant differentially expressed genes, Significance Analysis of Microarrays (SAM, version 3.02) were performed
|
| |
|
| Submission date |
Apr 27, 2015 |
| Last update date |
Apr 28, 2015 |
| Contact name |
Lin Shi |
| E-mail(s) |
[email protected]
|
| Organization name |
Xi'an Jiaotong University Health Science Center
|
| Department |
Department of Immunology and Microbiology
|
| Lab |
Key Laboratory of Environment and Genes Related to Diseases (Xi'an Jiaotong University), Ministry of Education of China
|
| Street address |
Yanta West Road No 76
|
| City |
Xi'an |
| State/province |
Shaanxi |
| ZIP/Postal code |
710061 |
| Country |
China |
| |
|
| Platform ID |
GPL14613 |
| Series (1) |
| GSE68285 |
miRNAs expression profile of the cumulus cell samples with PCOS and non-PCOS |
|
