|
| Status |
Public on Jun 25, 2015 |
| Title |
LIF 4 hour C |
| Sample type |
RNA |
| |
|
| Source name |
C2 myotubes 4 hours no treatment
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cultured C2 myotubes
agent: control
time: 4 hrs
|
| Treatment protocol |
not treated (C) or treated (E) with 10 ng/ml mouse LIF
|
| Growth protocol |
3 day differentiated C2C12 myotubes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the miRNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Whole-transcript expression profiling experiments were carried out by the Boston University Microarray Core Facility. The Affy standard WT Sense Target Labeling assay protocol was followed to synthesize biotinylated DNA.
|
| |
|
| Hybridization protocol |
Fragmented, biotinylated DNA samples were hybridized on a mouse Gene 1.0 ST array acorrding to manufacture's recommendation.
|
| Scan protocol |
A total of 6 array images were acquired by GeneChip Scanner 3000 TG and quality assessed by Affymetrix Expression Console (Santa Clara, CA).
|
| Description |
C2LIF4hC
|
| Data processing |
Gene expression signal values were generated by the Expression File Creator module of the GenePattern platform (Broad Institute, Cambridge, MA) and robust multi-array analysis algorithm (RMA) and quantile normalization was used for data preprocessing {Bolstad, 2003 #4365}. probe group file: MoEx-1_0-st-v1.r2.pgf meta-probeset file: Brainarray MoGene 1.0 ST custom CDF file v.16 was used for probe annotation {Allan, 2005 #4379}.
|
| |
|
| Submission date |
May 13, 2015 |
| Last update date |
Jun 25, 2015 |
| Contact name |
Robert William Jackman |
| E-mail(s) |
[email protected]
|
| Phone |
6173532719
|
| Organization name |
Boston University (BU)
|
| Department |
Health Sciences
|
| Street address |
635 Commonwealth Avenue/rm 444
|
| City |
Boston |
| State/province |
Massachusetts |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL19485 |
| Series (1) |
| GSE68827 |
Leukemia Inhibitory Factor in C26 Cancer Cachexia |
|
