|
| Status |
Public on May 16, 2015 |
| Title |
siKDM3A-B + E2 Replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
siKDM3A-B + E2 Replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
transfection: siKDM3A-B
agent: 10 nM E2
cell type: Human breast adenocarcinoma cell line
cell line: MCF-7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TRIzol reagent (Ambion, Life Technologies) following manufacturers instructions. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
According to manufacturer (Illumina) specifications.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Array 1
|
| Data processing |
Data was processed and analysed using R statistical software and BioConductor packages. Background corrected signal intensities were variance-stabilized and normlized using the 'vsn' package (Huber, W, von Heydebreck, A., Sueltmann, H., Poustka, A., and Vingron, M. Variance stabilization applied to microarray data calibration and to the quantification of differential expression. Bioinformatics: Vol. 18 suppl. 1 S96-S104 (2002)) .
|
| |
|
| Submission date |
May 15, 2015 |
| Last update date |
May 16, 2015 |
| Contact name |
Mark A Wade |
| E-mail(s) |
[email protected]
|
| Organization name |
Newcastle University
|
| Department |
Northern Institute for Cancer Research
|
| Street address |
Paul O'Gorman Building, Framlington Place
|
| City |
Newcastle Upon Tyne |
| State/province |
Tyne and Wear |
| ZIP/Postal code |
NE2 4HH |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE68918 |
Identification of KDM3A regulated genes in the ER positive breast cancer cell line MCF-7 |
|
