|
| Status |
Public on Jun 25, 2015 |
| Title |
phr123 triple mutant under -P condition repeat2 |
| Sample type |
RNA |
| |
|
| Source name |
phr123 triple mutant under -P condition
|
| Organism |
Oryza sativa |
| Characteristics |
tissue: Leaf
genotype: phr123 triple mutant
treatment: -P condition
|
| Treatment protocol |
Fourteen days plants growth under hydroponic +P condition (200 µM) were treated with –P (no phosphate) for another 7 days, shoot of plants from 3 biological repeats were sampled for Affymetrix microarray analysis.
|
| Growth protocol |
Hydroponic experiments were conducted using rice culture solution with NaH2PO4 (0 μM Pi). Nutrient solution was adjusted to pH 5.5 using 1 M NaOH and replaced every 2 days. Experiments were carried out in a greenhouse with a 12 h day (30º)/12 h night (22º) photoperiod, 200 μmol m-2 s-1 photon density, and 60% humidity.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5 ug total RNA (GeneChip® 3’ IVT Express Kit User Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rice Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the affymetrix Genechip Scanner 3000.
|
| Description |
Gene expression data from phr123 mutant under -P condition
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
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| |
|
| Submission date |
May 18, 2015 |
| Last update date |
Jun 25, 2015 |
| Contact name |
Guo meina |
| E-mail(s) |
[email protected]
|
| Phone |
(86)-18858104918
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yu Hang Tang Road
|
| City |
Hangzhou |
| ZIP/Postal code |
310058 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE69010 |
Expression data from -Pi treatment between WT, phr1, phr3 and phr1phr2phr3 triple mutant |
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