|
| Status |
Public on Jul 09, 2015 |
| Title |
KMS-34/Cfz_rep1_261893 |
| Sample type |
RNA |
| |
|
| Source name |
KMS-34/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: KMS-34/Cfz
cell type: multiple myeloma
|
| Treatment protocol |
KMS-11/Cfz and KMS-34/Cfz cells were profiled for gene expression after 1 week of growth in the absence of carfilzomib together with parental KMS-11 and KMS-34 cells which had not been selected in the drug.
|
| Growth protocol |
Cells for gene expression profiling were cultured in RPMI 1640 with GlutaMAX (Life Technologies) supplemented with 4% fetal bovine serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the miRNeasy mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared by Expression Analysis, Inc. (Durham, NC).
|
| |
|
| Hybridization protocol |
Hybridization of cRNA on Affymetrix GeneChip Human Genome U133 Plus 2.0 arrays was performed by Expression Analysis, Inc.
|
| Scan protocol |
Scanning of GeneChip arrays was performed by Expression Analysis, Inc.
|
| Description |
Gene expression data from KMS-34/Cfz multiple myeloma cells resistant to 12 nM carfilzomib
|
| Data processing |
The data were analyzed with GeneSpring software (Agilent Technologies) using default settings and the RMA normalization and summarization algorithm.
|
| |
|
| Submission date |
May 20, 2015 |
| Last update date |
Jul 10, 2015 |
| Contact name |
Robert Hawley |
| Organization name |
George Washington University
|
| Department |
Anatomy and Regenerative Biology
|
| Street address |
2300 I Street NW, Suite 431
|
| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20037 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE69078 |
Comparison of the gene expression profiles of carfilzomib-resistant derivatives versus parental human myeloma cell lines |
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