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Status
Public on Feb 21, 2004
Title
Human myometrial cells stably-transfected with the ATF2 gene
Sample type
RNA
Source name
human myometrium
Organism
Homo sapiens Extracted molecule
total RNA
Description
Human myometrial cells taken from biopsies of pre-menopausal non-cancerous non-pregnant uteri were cultured in complete MEM D-valine medium plus 10% FCS, and stably-transfected with the pcDNA3.1/V5-His TOPO vector (Invitrogen) harbouring the ATF2 gene. Total RNA was extracted using TRI-reagent (Sigma) and RNeasy columns (Qiagen), and 10 ug used in target preparation for array hybridization.
Submission date
Feb 19, 2004
Last update date
May 28, 2005
Contact name
Jarrod Bailey
E-mail(s)
[email protected] Phone
(+44) 191 2228748
Fax
(+44) 191 2225066
Organization name
University of Newcastle upon Tyne
Department
School of Surgical and Reproductive Sciences
Street address
Leech Building, Medical School
City
Newcastle upon Tyne
State/province
Tyne and Wear
ZIP/Postal code
NE2 4HH
Country
United Kingdom
Platform ID
GPL96 Series (1)
GSE1059 Myometrial cells expressing CREB, CREM alpha, CREM tau2alpha, ATF2 or the ATF2-small gene
Data table header descriptions ID_REF
VALUE Scaled signal intensity data
ABS_CALL Absolute detection call
Data table ID_REF VALUE ABS_CALL AFFX-BioB-5_at
190.4
P
AFFX-BioB-M_at
263.8
P
AFFX-BioB-3_at
165.2
P
AFFX-BioC-5_at
464.5
P
AFFX-BioC-3_at
327.9
P
AFFX-BioDn-5_at
420.2
P
AFFX-BioDn-3_at
2686
P
AFFX-CreX-5_at
4732.1
P
AFFX-CreX-3_at
7202.8
P
AFFX-DapX-5_at
4.7
A
AFFX-DapX-M_at
9
A
AFFX-DapX-3_at
4.5
A
AFFX-LysX-5_at
2
A
AFFX-LysX-M_at
16
A
AFFX-LysX-3_at
2.5
A
AFFX-PheX-5_at
2
A
AFFX-PheX-M_at
8.7
A
AFFX-PheX-3_at
14.3
A
AFFX-ThrX-5_at
7.7
A
AFFX-ThrX-M_at
24.3
A
22283 393 Kbytes .Supplementary data files not provided
