|
| Status |
Public on Feb 08, 2017 |
| Title |
WT_ovary_Rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Whole ovary
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: w[1118]
tissue: ovarian tissues
|
| Growth protocol |
Drosophila animals were grown on cornmeal agar in 27 degrees Celsius.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
GeneJET RNA purification kit (Thermo Scientific, K0732)
RNA libraries were prepared for sequencing using Illumina TruSeq RNA v2 protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
Illumina Casava1.8.2 software used for basecalling.
Reads were mapped to Drosophila melanogaster genome BDGP release 5/dm3 using STAR (STAR_2.3.0e_r291)
Gene level FPKM was estimated using cufflinks (v2.1.1) with GTF file from Ensemble release 74
Genome_build: D. melanogaster BDGP release 5/dm3
Supplementary_files_format_and_content: tab-delimited text files include gene level FPKM valuse for each Sample
|
| |
|
| Submission date |
Jun 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
David Finkelstein |
| E-mail(s) |
[email protected]
|
| Phone |
9014953931
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Computational Biology
|
| Street address |
332 N. Lauderdale St.
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL17275 |
| Series (1) |
| GSE69722 |
piwi mutations and c-Fos overexpression on Drosophila ovarian germline |
|
| Relations |
| Reanalyzed by |
GSM3275876 |
| BioSample |
SAMN03766461 |
| SRA |
SRX1054683 |
